ack at it. CYP P450 in humans is critical in drug activity. “Why alcohol is bad for your liver – makes drugs work too quickly / metabolize too quickly / get destroyed by P450 too quickly” – P450 rushes towards it to destroy it.
“Why grapefruit juice is bad for your liver – inhibits P450, causes it to NOT breakdown drugs like it’s supposed to…”
TONS of stuff on it from a Drug POV. I guess I’ll have to learn a little about this side of things to find out what I want.
–because complex patterns of cooperativity are observed with several P450s. —
“Cytochrome P450 (P450) enzymes catalyze a variety of oxidation and some reduction reactions, collectively involving thousands of substrates. ”
“A general chemical mechanism can be used to rationalize most of the oxidations and involves a perfenyl intermediate (FeO3+) and odd-electron chemistry, i.e. abstraction of a hydrogen atom or electron followed by oxygen rebound and sometimes rearrangement. This general mechanism can explain carbon hydroxylation, heteroatom oxygenation and dealkylation, epoxidation, desaturation, heme destruction, and other reactions. Another approach to understanding catalysis involves analysis of the more general catalytic cycle, including substrate specificity,
because complex patterns of cooperativity are observed with several P450s.
Some of the complexity is due to slow conformational changes in the proteins that occur on the same timescale as other steps.